FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6659515
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCG97690.14669236423373175TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG12650.036.82150745
GATCGGA5100.033.56471
CTCGTAT16000.029.10872541
TCTCGTA14500.028.70560840
CGTATGC16350.028.48645643
CGATGTA16100.027.94800833
ATCGGAA17200.027.8926981
ACCGATG16550.027.7322731
CCGATGT16750.027.26681732
GTCACCG17400.027.0240428
CGTCTGA17750.026.99797615
TCGGAAG17650.026.9127732
TCGTATG17350.026.8439842
ACACGTC17850.026.72008512
CACCGAT17200.026.68424830
ATCTCGT15700.026.51154939
TATCTCG15900.026.03656638
ACGTCTG18650.025.69454814
TCACCGA18300.025.44910429
CGGAAGA18950.025.1856923
Produced by FastQC (version 0.11.5)