FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10138903
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC11400.032.74244745
ATCGGAA11700.032.6451071
CTCGTAT11650.031.653843
TCTCGTA11750.031.38440342
TCGGAAG12750.029.098812
TATCTCG12850.028.6976640
TCGTATG13200.028.61826344
ATCTCGT13700.027.08141141
ACACGTC14250.026.66694612
CGTCTGA14150.026.5375915
CGGAAGA15650.023.9940533
ACGTCTG16500.022.75799614
CACGTCT18600.020.79299413
GCACACG18950.020.40895310
CACACGT20850.018.87267511
GTCACAC28800.013.741240528
CGTTGGA25800.013.0994541
AGAGCAC34150.011.65424257
AACGGGA22850.011.3165258
GAAGAGC35900.011.1487725
Produced by FastQC (version 0.11.5)