FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18909557
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG14150.032.11851545
GATCGGA13550.030.7646981
CTCGTAT24800.018.50771341
CGTATGC25600.017.928443
TCGTATG25800.017.70315442
ATCGGAA27250.017.116861
TCGGAAG28350.016.3546922
TCTCGTA22500.016.29973440
ACACGTC28400.016.23904212
CGTCTGA30900.015.36216315
CGGAAGA31350.015.14862253
TCACGAT24400.014.47710834
ATCTCGT25450.014.41037439
CATCACG31350.014.21018232
CACGTCT33600.014.19454713
ACGTCTG33600.014.12759114
GCACACG38500.012.15506810
ACGATCT29050.011.77301936
GTATGCC40600.011.69272644
ATCACGA38800.011.59765733
Produced by FastQC (version 0.11.5)