FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005310455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129640101
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCG74424175.740829375009512TruSeq Adapter, Index 8 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC44172633.4073276447077125TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA4707300.044.5765531
TATGCCG8784100.044.04316745
CGTCTTA160350.033.01275315
CCCGTCA173100.029.50545125
ATCGGAA13423800.029.1801131
ACACGTC13470850.029.1633812
TCGGAAG13419950.029.1324712
CACGTCT13521200.029.07241813
CGGAAGA13463050.029.0709613
GCACACG13562750.029.06231110
CCGTCAC178400.029.03272626
CACACGT13590000.029.00747711
CGTCTGA13372950.028.98539515
ACGTCTG13389400.028.96122214
TCCCGTC180950.028.95831924
CTCGTAT13404500.028.89852341
TCTCGTA13398300.028.8919340
CGTATGC13464650.028.88079343
ATCTCGT13428000.028.87068639
TCGTATG13463800.028.86776242
Produced by FastQC (version 0.11.5)