FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14170004
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCG473890.3344318039712621TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG58850.040.2615445
ATCGGAA68300.035.8424951
TCTCGTA66000.035.5925340
CTCGTAT66650.035.54950741
GTCACGC67550.035.47358728
CGCCAAT67450.035.35901632
TATCTCG66850.035.10457638
CGTATGC68650.035.03850643
TCGGAAG70150.034.960542
ACGCCAA68050.034.8158131
TCGTATG68950.034.75551642
ACACGTC70650.034.68102612
ATCTCGT68400.034.30956339
CGTCTGA71300.034.049315
AGTCACG68900.034.02768727
CGGAAGA72650.033.8504033
ACGTCTG72150.033.67934414
CACGTCT74900.032.6831113
GCACACG77900.031.42445410
CACACGT79900.030.58153711
Produced by FastQC (version 0.11.5)