FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6688059
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCG266330.3982171807993919TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG31100.041.02028745
ATCGGAA35750.036.277241
CGCCAAT35200.035.92141732
GTCACGC35850.035.39563828
TATCTCG36300.035.14436738
TCTCGTA36300.035.08185640
CTCGTAT36400.035.04781741
TCGGAAG37200.034.967522
CGTATGC36550.034.780643
ACGCCAA36300.034.7709131
ATCTCGT36900.034.5729139
ACACGTC37200.034.47322512
TCGTATG36950.034.46446642
CGTCTGA37300.034.32048815
AGTCACG37350.033.91439427
CGGAAGA38350.033.8602643
TACGGCG803.6379788E-1233.748496
ACGTCTG38800.033.16762514
CACGTCT39200.032.77178613
GCACACG41150.031.54685210
Produced by FastQC (version 0.11.5)