FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15406275
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG8500.030.97347834
GTCCCGA9850.026.49984735
ACCCGTC10950.024.8604131
TCCCGAT8550.024.4758936
CCCGATC8600.023.5487137
CGTATGC11300.023.29424745
CTCGTAT11100.023.10583943
TCGTATG11450.022.79267344
ATCGGAA11700.022.736421
ACACGTC11900.022.49750112
CCGATCT9100.022.00747938
CACCCGT12250.021.85478630
TCTCGTA9550.021.43817342
CGTCTGA12900.020.40470915
TCGGAAG13850.019.335192
TCACCCG13850.019.33004429
CCCGTCC14650.018.5829432
CCGTCCC14850.018.18351433
ACGTCTG15600.017.30576914
ATCTCGT12100.016.92021241
Produced by FastQC (version 0.11.5)