FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15066417
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCG951960.6318423285377007TruSeq Adapter, Index 7 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC150800.10009015414879331TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG108550.042.4922845
CTCGTAT125450.036.80502741
ATCGGAA127500.036.7972221
TCTCGTA125550.036.7040240
TCGTATG126000.036.62663342
CGTATGC127050.036.46573643
TCGGAAG129850.036.230682
ATCTCGT127800.036.1458639
ACACGTC130500.035.94605612
CGTCTGA131150.035.66497815
CATCTCG129350.035.66041638
CGGAAGA134100.035.2330783
ACGTCTG133500.034.96975314
CACGTCT136350.034.46982213
GCACACG136950.034.25331510
CACACGT138950.033.88982411
GATCGGA21600.033.889531
GTATGCC137750.033.71486344
GATCATC150200.031.0240935
AGTCACC155300.030.17772927
Produced by FastQC (version 0.11.5)