FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8296238
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC7950.028.30289545
TCTCGTA8350.025.60094842
CTCGTAT8350.025.60048743
AGTTCCG8650.025.2290733
CCGTATC9050.024.11658937
TCCGTAT9100.023.73596436
TATCTCG9150.023.36247440
ACACGTC9650.023.30944612
GTTCCGT9450.022.85547834
TCGTATG9950.022.38773244
TCGGAAG10150.022.3829442
CGTATCT9700.022.26829738
ATCGGAA9900.022.206761
ATCTCGT9700.021.80621541
TTCCGTA10350.021.52146735
CGTCTGA10700.021.0220715
CGGAAGA11800.019.4437483
ACGTCTG12050.018.10689414
CACGTCT13000.017.12975313
GCACACG13450.016.72387710
Produced by FastQC (version 0.11.5)