FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4373649
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG3049876.9732847789111565TruSeq Adapter, Index 20 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATT56310.12874832891253962TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA412800.044.526821
ATCGGAA413100.044.432022
TCGGAAG413200.044.3821263
ACACGTC409950.044.3285913
CGTCTGA410200.044.18689316
CGGAAGA414450.044.1706054
ACGTCTG411600.044.08579615
GCACACG413050.044.0637811
CACACGT412500.044.0570812
CACGTCT412550.044.05467614
TCGTATG341700.043.92031545
TCTCGTA348850.043.858643
CTCGTAT348850.043.81990444
TATCTCG352750.043.8010641
AGTCACG409600.043.80069728
GTCACGT399950.043.7886829
ATCTCGT347500.043.73114842
ACGTGGC378650.043.70332332
CGTGGCC377750.043.63471633
TCACGTG399450.043.584430
Produced by FastQC (version 0.11.5)