FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13977155
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGC144610.10346168444150473TruSeq Adapter, Index 18 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC20600.036.5863645
ACGTCCG24400.030.88627431
CGTCCGC24850.030.32696732
GATCGGA9100.029.6581021
GTCCGCA26000.028.89998833
TCCGCAC26050.028.84451734
CTCGTAT27450.027.6204543
ATCGGAA28150.027.4734881
TCTCGTA25650.026.7509142
TCGTATG28300.026.63176744
ACACGTC29250.026.60878612
CGCACAT26550.026.18285236
TCGGAAG29800.025.8199022
GTCACGT29250.025.61049828
CGTCTGA30950.025.07455815
CCGCACA30000.024.89712335
AGTCACG30150.024.84609427
ATCTCGT28300.024.08740641
TCACGTC31450.023.96255929
ACGTCTG32600.023.80544514
Produced by FastQC (version 0.11.5)