FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7661084
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATG1742032.273868815431341TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA184400.044.5361141
ATCGGAA188300.043.6014632
TCGGAAG189500.043.3016173
CGGAAGA192000.042.6088874
ACACGTC190000.042.5718813
GTCACGA188550.042.3275929
CGTCTGA191000.042.30187216
TCTCGTA189000.042.28550743
TATCTCG189150.042.24007441
TCGTATG190000.042.19321445
CTCGTAT189750.042.14208644
ACGTCTG191850.042.13790515
CACGAGT190350.042.0460831
ACGAGTG191600.042.0186632
ATCTCGT190500.041.9525542
AGTCACG190400.041.88086328
CACGTCT193350.041.84591314
GCACACG194000.041.72890511
CACACGT194200.041.65117312
CGAGTGG195500.041.15688333
Produced by FastQC (version 0.11.5)