FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9111181
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGC122700.1346696986921893TruSeq Adapter, Index 18 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC17400.035.3006645
GATCGGA8250.033.026011
ACGTCCG21300.028.94373931
CGTCCGC21500.028.6735532
TCCGCAC21950.027.88070534
GTCCGCA22250.027.40366633
CTCGTAT22850.026.48759843
ATCGGAA23650.026.3738351
ACACGTC23900.025.98290812
TCGTATG23750.025.38870644
CGTCTGA24700.025.23244515
CCGCACA24050.025.16569735
TCGGAAG25000.025.113242
TCTCGTA21000.024.96394242
AGTCACG24650.024.82724427
TCACGTC25250.024.59412429
CGCACAT21200.024.51590336
ACGTCTG25650.024.21019414
GTCACGT25250.024.14804828
ATCTCGT22100.023.72152341
Produced by FastQC (version 0.11.5)