FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6905262
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGC173450.25118525553411297TruSeq Adapter, Index 16 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC22500.040.90244345
CGTCCCG23100.039.9342734
GTCCCGA23700.038.63847435
CTCGTAT23950.037.95633743
CCGATCT20350.037.92307338
ATCGGAA24950.037.4661561
TCGGAAG25150.037.3046072
CCCGATC20800.037.21106337
TCCCGAT20900.037.0330236
ACCCGTC25050.036.73579431
TCTCGTA21000.036.53789542
TCGTATG25050.036.37941744
ACACGTC25850.036.2076512
CCGTCCC25850.036.034133
CGTCTGA25750.035.99823815
CACCCGT25650.035.96419530
TCACCCG25650.035.7887629
ATCTCGT21850.035.42314541
CGGAAGA26750.035.2415243
CCCGTCC26500.034.8955332
Produced by FastQC (version 0.11.5)