FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34350217
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA80000.013.8184211
ACGGGAT51450.012.4198039
AACGGGA70450.012.2639598
GAAACGG88500.011.5677596
AAACGGG87850.011.4996617
GGAAACG94800.010.6816515
CGGGATT52650.010.51254110
ACAGGCG132700.08.01960518
ACCGCGC30950.07.996451431
CGGGAAT35050.07.895653210
ACGCCCG26150.07.743456434
CCGAGTA66650.07.7312762
GCGTGAG90750.07.561705622
CACCGCG35150.07.553043430
CCCAAGT161850.07.44231461
CCGGCTA50550.07.43315638
ACCACGC78000.07.41316731
CCCGAGT87800.07.3083151
CGGCTAA50450.07.269560339
CGCCCGG50000.07.244733335
Produced by FastQC (version 0.11.5)