FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9134266
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCG138270.1513750530146593TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG17350.040.3307745
CGCCAAT19450.035.97609332
GTCACGC19700.035.63375528
CTCGTAT19750.035.54393441
ACGCCAA20250.034.44370731
ATCGGAA20700.034.2483331
CGTCTGA20800.034.07341815
TCGTATG20750.033.93940442
TATCTCG20500.033.91446738
AGTCACG20800.033.8574527
ACACGTC21050.033.5627812
CGTATGC20950.033.50800343
TCTCGTA20750.033.50604240
TCGGAAG21450.033.150862
ATCTCGT21600.032.1873439
CGGAAGA22500.031.3024163
ACGTCTG23050.031.23543214
CACGTCT24300.029.72207513
GCACACG25150.028.0913110
CACACGT26300.027.11963811
Produced by FastQC (version 0.11.5)