FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5610897
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG2000.025.8767731
CGTCCGC2150.023.02503432
GTCCGCA2800.019.28686133
CGTATGC3200.017.58042345
TCCGCAC3100.016.69483834
CTCGTAT3450.015.6542243
GATCGGA1602.9305556E-715.4865091
CCGCACA3550.015.21244235
TCGTATG3500.014.78764744
CGCACAT3700.013.98806636
TCTCGTA3650.013.56365942
AGTCACG3950.013.10216127
CGTTGGA12400.012.7161951
CACGTCC4450.012.64131430
ATCGGAA3900.012.1292931
AACGGGA11350.012.0906438
ACACGTC4000.011.81057112
CGTCTGA4200.011.78378915
GTCACGT4400.011.76216728
TCACGTC4600.011.73993329
Produced by FastQC (version 0.11.5)