FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16571495
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC7450.018.72307645
CTCGTAT7300.016.33403643
ATCGGAA8850.012.4816541
TCGTATG9400.012.4456844
TCTCGTA7550.012.21758242
CGTTGGA35250.010.9998991
TCGGAAG10400.010.819462
ACGGGAT23100.010.6161779
ACACGTC11050.09.97631912
AACGGGA32150.09.867158
CGGGATT22100.09.77310510
AAACGGG39700.09.634127
CGTCTGA11500.09.39031115
GAAACGG40700.09.342136
GGAAACG39600.09.2608035
ATCTCGT10300.09.17400841
TCGCGTT2751.2356088E-48.99948
ACGTCTG13300.08.96521714
CGGCTAA22050.08.46984639
CGCCCGG22100.08.34909435
Produced by FastQC (version 0.11.5)