FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23430699
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA55800.012.3043051
ACGGGAT37050.011.2934059
AACGGGA50100.011.2254158
AAACGGG60100.011.0045687
GAAACGG66150.010.3380876
GGAAACG65800.010.0853865
CGGGATT35700.09.89308210
ACGCCCG17350.08.94707934
CGTATGC9400.08.61557145
ACCGCGC20700.08.47750431
CACCGCG22400.08.13543330
ACACGTC11600.07.951078412
CTCGTAT9400.07.897623543
CCGGCTA33050.07.759976438
GCGTGAG62750.07.63598622
CGTGAGC65750.07.561287423
ACAGGCG89500.07.54040518
CGGCTAA32800.07.40760639
CGGGAAT24600.07.40713210
CCCAAAG258050.07.3321491
Produced by FastQC (version 0.11.5)