FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7879503
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGC877301.1133950961120263TruSeq Adapter, Index 27 (97% over 43bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATG514960.6535437577725397TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC108000.042.0446945
GATCGGA74000.034.5748441
TCGGAAG162300.028.1019172
ATCGGAA162950.028.0302641
CTCGTAT160700.027.90400943
TCGTATG161100.027.83719844
CGTCTGA167000.027.36122515
CACGTCT167650.027.22812813
CGGAAGA167850.027.1599983
ACGTCTG169250.026.97073214
CACACGT169900.026.907111
ACACGTC170550.026.80472412
TCTCGTA152450.026.75716242
TATCTCG152550.026.73894140
GTCTGAA174350.026.19486616
TCTGAAC174600.026.08004617
CCAGTCA174650.025.98273825
GCACACG176350.025.9102210
CAGTCAC175350.025.89266626
AGAGCAC176500.025.8761067
Produced by FastQC (version 0.11.5)