FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18153344
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCG1579530.8701041527114783TruSeq Adapter, Index 7 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC390340.2150237443856074TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG179650.042.96785445
GATCGGA50800.041.2477461
ATCGGAA228800.034.4169351
CTCGTAT225700.034.17113541
TCGGAAG230500.034.1378442
ACACGTC229350.034.06743212
TCTCGTA216350.034.0292240
CGTATGC227800.033.96476443
TCGTATG227900.033.93011542
CGTCTGA230250.033.83833315
CGGAAGA233100.033.7025683
ACGTCTG233450.033.48839614
ATCTCGT220550.033.4307339
CACGTCT235200.033.28705213
CATCTCG224100.032.8710338
GCACACG238750.032.77307510
CACACGT242400.032.3354511
GTATGCC240250.032.15785244
GATCATC245500.030.05159835
AGTCACC261350.029.79649227
Produced by FastQC (version 0.11.5)