FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18291788
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC8550.021.84014545
CTCGTAT10350.018.25998143
TCGTATG10850.017.83250244
TCTCGTA10150.015.73776242
ATCGGAA12800.014.8349861
ACACGTC13400.014.43821512
TCGGAAG13500.013.9984012
CGTCTGA15250.012.83421315
ATCTCGT12350.012.75185841
CGTTGGA42350.012.0101091
ACGTCTG17900.010.68281614
AAACGGG47850.010.6724417
CGGAAGA18500.010.5798433
AACGGGA38450.010.5316398
GGAAACG49600.010.3415325
ACGGGAT29000.010.3174759
GAAACGG48650.010.2657336
CACGTCT20850.09.71082913
CGGGATT28450.09.56803910
AACGGGT10550.08.7428148
Produced by FastQC (version 0.11.5)