FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8413503
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC8650.032.25700445
CTCGTAT10300.026.65269343
TCTCGTA10300.026.65269342
TCGTATG10850.025.30163444
ATCGGAA11150.024.8212091
ACACGTC12050.023.52654512
CGTCTGA12000.023.24957515
ATCTCGT11950.022.59587941
TCGGAAG12450.022.2286432
CGGAAGA13800.020.05413
ACGTCTG13950.019.99963614
AATCTCG14700.018.67479340
CACGTCT15700.018.05699513
CGTCCGA750.001111108317.9997796
GCACACG16050.017.3828610
GATCGGA3800.017.1714531
CACACGT18700.015.40078911
CGTTGGA20100.013.3212071
AAACGGG22350.011.576977
AGTCAAC24100.011.48319433
Produced by FastQC (version 0.11.5)