FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7361926
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCG931341.2650765574117424TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG115600.042.99738345
ATCGGAA119150.042.7834431
TCGGAAG119800.042.4247552
ACACGTC119300.041.7916212
CGGAAGA121700.041.5957453
CGCCAAT117150.041.5610632
CGTATGC119850.041.47265643
GTCACGC118500.041.44805528
CGTCTGA119900.041.39512315
CTCGTAT119350.041.30676341
AGTCACG119150.041.2597127
TCGTATG119750.041.18785542
TCTCGTA106500.041.15698240
ACGCCAA118850.041.15589531
ACGTCTG120950.041.09156414
TATCTCG106250.040.9980238
ATCTCGT107000.040.87887239
CACGTCT122750.040.58036813
GCACACG124950.040.27974310
GTATGCC124300.040.04221744
Produced by FastQC (version 0.11.5)