FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14085684
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATGC167220.1187162795928121TruSeq Adapter, Index 22 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG24350.038.71645432
CGTACGT24400.038.6371133
CGTATGC25500.037.3297245
ACCGTAC26550.035.59280431
GTACGTA27400.034.24254234
CTCGTAT28000.033.9093543
TCTCGTA26600.032.31073442
TCGTATG29300.032.25790444
ATCGGAA30150.031.967161
CGTAATC26750.031.87835737
TCGGAAG30450.031.7139952
ACACGTC30700.031.5861212
GTCACCG30350.031.4314628
CACCGTA30900.030.72637630
TACGTAA31050.030.72449335
ACGTAAT28600.030.20964836
CGTCTGA31850.030.09244715
ATCTCGT29550.029.00898741
CGGAAGA33750.029.007593
ACGTCTG33950.028.7612214
Produced by FastQC (version 0.11.5)