FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10049906
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCG990620.9857007617782694TruSeq Adapter, Index 1 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC138080.13739431990707177TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG122600.041.86313245
GATCGGA19600.038.7110061
ATCGGAA137350.038.062221
TCGGAAG137750.037.9065552
CGGAAGA139500.037.331993
CTCGTAT139000.036.77544841
CGTATGC139700.036.70391543
CGTCTGA140400.036.56826415
ACACGTC140500.036.5582512
TCTCGTA133500.036.53762440
TCGTATG140850.036.4202142
ATCTCGT133950.036.31482339
ACGTCTG142000.036.1403814
TCACGAT135300.036.01917634
CATCACG142450.035.86952632
CACGTCT143950.035.697713
GCACACG145750.035.36577210
CACACGT145900.035.2822711
ACGATCT137400.035.23923536
CACGATC137750.035.14987635
Produced by FastQC (version 0.11.5)