FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11792470
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGC432850.36705626556607734TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC55450.040.085845
ATCGGAA59900.038.1299321
ACACGTC59250.037.93160612
GTCACGA58800.037.7253228
TCTCGTA53200.037.59458542
TCGGAAG60500.037.594122
CTCGTAT58850.037.42582743
CGTCTGA59650.037.37552615
TATCTCG53850.037.09902240
TCGTATG59700.037.00601644
CGGAAGA61600.036.995843
AGTCACG60450.036.77002727
ACGAGTG60600.036.61221731
CACGAGT60650.036.5367230
ACGTCTG62550.035.9304214
ATCTCGT56100.035.6511941
CACGTCT64450.034.83627713
GCACACG65150.034.49651310
CACACGT65350.034.49421311
CGAGTGG66450.033.42288232
Produced by FastQC (version 0.11.5)