FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9936311
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG9200.030.32421545
GATCGGA7050.029.087161
ACGGCTA12300.022.3164831
CGGCTAC13150.020.87397232
CTCGTAT13600.020.34831641
TCGTATG14100.019.62674342
ATCGGAA14700.019.3153361
CGTATGC14450.019.30657243
GTCACGG15000.018.89921228
AGTCACG15550.018.51938427
ACACGTC15250.018.44046412
TCACGGC15450.018.34930629
TCTCGTA12600.017.8563140
TCGGAAG15800.017.8075082
CGTCTGA16500.017.04345915
CACGGCT17450.016.24623330
ACGTCTG18050.016.2030914
CACGTCT17950.016.16802413
CGGAAGA18100.015.9177493
ATCTCGT14100.015.79713539
Produced by FastQC (version 0.11.5)