FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23926908
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCG311990.13039294504747542TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG263700.041.9110445
GATCGGA68250.040.7111931
ATCGGAA348950.035.544191
TCGGAAG350850.035.3445052
CGGAAGA356450.034.810333
ACACGTC358300.034.45304512
CGTCTGA358900.034.3014215
CATCTCG280750.034.0274738
ACGTCTG365050.033.7913414
CACGTCT366550.033.70215613
GTATGCC334550.033.37734244
GCACACG370950.033.3327310
CACACGT375600.032.9080811
CGTATGC342450.032.58771543
CTCGTAT350950.031.61860541
TCGTATG353850.031.55055442
CTTAGGC305800.031.54053532
TCTCGTA351900.031.48934740
ATCTCGT355500.031.10039139
ACTTAGG316500.030.51086831