FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16448425
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGC177960.10819272969904413TruSeq Adapter, Index 20 (97% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC25150.035.0756245
CTCGTAT28550.030.73336843
TCGTATG28600.030.37236244
ATCGGAA29500.030.130381
TCTCGTA27650.029.94362342
TATCTCG27850.029.88897540
TCGGAAG30950.028.789562
ATCTCGT29650.027.99969741
AGTCACG31650.027.79313527
CGTCTGA32500.027.75846915
ACACGTC32350.027.7480912
GTCACGT31800.027.73278228
ACGTCTG34700.025.73922714
CGGAAGA35350.025.5880473
TCACGTG35800.024.63414629
ACGTGGC36100.024.4924231
CACGTCT37600.023.93352313
CGTGGCC37450.023.68118132
GCACACG38750.023.51352910
CACACGT41900.021.58472411
Produced by FastQC (version 0.11.5)