FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38325572
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC22200.020.06891445
CTCGTAT21800.019.40289543
ATCGGAA25100.017.9484121
TCTCGTA20550.017.40806442
ACACGTC27600.016.4665212
TCGTATG27200.016.2126144
TCGGAAG28050.016.1277372
CGTCTGA29200.015.33309215
GTCACGT30550.014.95012928
AGTCACG29800.014.94889327
ATCTCGT24450.014.63137541
AACGATC24950.014.24781737
CGTTGGA82450.013.0862321
ACGTCTG35250.012.63762914
CGGAAGA36800.011.9863913
CACGTCT41050.011.45493513
CACGTGA42800.011.03914930
TCACGTG42400.010.93105329
ACGGGAT61850.010.8037939
AAACGGG97350.010.7467357
Produced by FastQC (version 0.11.5)