FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7572483
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG170070.22458947745409266TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA19750.041.387671
TCTCGTA22050.036.32404343
TATCTCG22250.036.09960641
CTCGTAT22450.035.97915344
ACACGTC22850.035.1512313
TCGTATG22800.035.13079545
ATCTCGT23100.034.67386242
TCGGAAG23700.034.6550143
ATCGGAA23800.034.6110422
GTCACGT23400.033.84450529
CGTCTGA23800.033.84266716
CGGAAGA24500.033.5229844
AGTCACG23750.033.25101528
ACGTCTG25050.032.06409515
ACGTGGC25100.031.82241832
GCACACG26050.031.17828611
CACGTCT25950.030.95204414
CGTGGCC25750.030.93216733
TCACGTG26950.029.46980930
CACACGT27700.029.1586512
Produced by FastQC (version 0.11.5)