FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13917221
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCG906490.6513441153230232TruSeq Adapter, Index 8 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC358020.2572496333858606TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG100850.042.61478845
GATCGGA42000.040.7986451
TCTCGTA137700.030.99829740
ATCGGAA140300.030.9909571
CTCGTAT138200.030.9028741
CGTATGC140050.030.76779243
TCGGAAG140950.030.762572
ACACGTC140800.030.72788812
TCGTATG141000.030.52857642
CGTCTGA141700.030.48519715
ATCTCGT140850.030.30428139
CGGAAGA143300.030.2894943
ACGTCTG143950.029.94618214
CACGTCT145700.029.80257813
GCACACG147550.029.4289110
AATCTCG146200.029.19470838
CACACGT150150.028.94928611
GTATGCC150150.028.7877744
GTCACAC160100.026.84154128
AGAGCAC169300.025.69097
Produced by FastQC (version 0.11.5)