FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45067372
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG32450.030.50652345
GATCGGA24700.021.8806611
CTCGTAT46800.021.34535841
CGTATGC47800.020.85117543
TCGTATG49700.020.09947442
TATCTCG46150.019.93957938
TCTCGTA46650.019.87055440
ATCGGAA53650.018.2585241
ACACGTC57100.017.53464512
CGTCTGA57750.017.41520515
ATCTCGT54500.017.17359439
TCGGAAG59050.016.7664642
ACGTCTG65900.015.363852514
CGGAAGA68900.014.7614143
CACGTCT72700.014.236279513
GTATGCC78550.013.17547944
GCACACG78450.013.1067910
CGTTGGA99950.012.12120151
CACACGT87200.011.843204511
CACTAGC93400.011.05708930
Produced by FastQC (version 0.11.5)