FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13705379
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG9250.028.21518931
GTCCGCA9650.027.04574833
CGTATGC10000.026.99891945
CGTCCGC10050.025.96920432
TCCGCAC10250.025.6821834
CTCGTAT11700.022.6917343
TCTCGTA12250.021.85658642
CGCACAT12250.021.4891736
TCGTATG12600.020.89232644
GTCACGT13200.020.1130528
ATCGGAA13100.020.1033421
ATCTCGT13400.019.98083541
AGTCACG13550.019.59352727
CCGCACA13600.019.5214935
TCGGAAG13750.018.9861832
ACACGTC14500.018.77545412
CGTCTGA14750.017.99914715
TCACGTC14850.017.57524729
CACGTCC15550.017.36283930
CATCTCG15700.017.34038540
Produced by FastQC (version 0.11.5)