FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26725803
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC14800.020.06438345
CTCGTAT14650.019.34846343
TCGTATG16250.018.13558244
AGTTCCG16800.017.94431933
ATCGGAA17300.017.030851
CCGTATC15750.016.71194537
TCCGTAT15900.016.55431636
TCTCGTA15500.016.400542
TATCTCG16450.015.86406940
TCGGAAG19000.015.6345382
CGTCTGA21550.014.92746615
ACACGTC20700.014.779736512
GTTCCGT20050.014.24995334
TTCCGTA21100.013.86069635
CGTTGGA61300.012.9033971
ATCTCGT19850.012.69310341
CGTATCT20500.012.62018438
ACGGGAT41100.012.53406059
AACGGGA55900.012.1935178
ACGTCTG25150.012.16461214
Produced by FastQC (version 0.11.5)