FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9722713
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC4735784.8708421198897875TruSeq Adapter, Index 6 (100% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCG1581841.626953299968846TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA507150.044.769711
TATGCCG209900.041.36514745
CGGAAGA713900.031.718744
TCGGAAG715000.031.7111843
ACACGTC709550.031.66712413
ATCGGAA717000.031.663692
CGTCTGA709800.031.65280516
CTCGTAT703350.031.61858642
ACGTCTG711550.031.59392715
CACGTCT713100.031.5788914
TCTCGTA704350.031.5735341
TATCTCG704800.031.5343839
TCGTATG707450.031.53393443
GTCACGC705800.031.50342629
ATCTCGT705650.031.49288440
CGTATGC709100.031.47276544
CACACGT714650.031.4379912
AGTCACG702450.031.42368528
CGCCAAT709150.031.40047633
ACGCCAA709950.031.37143332
Produced by FastQC (version 0.11.5)