FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310507

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310507
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9145856
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATGC129530.14162698384929742TruSeq Adapter, Index 22 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG19100.036.86703532
CGTATGC19550.036.3686345
CGTACGT19450.036.20599433
GTACGTA20900.033.91013734
ACCGTAC20750.033.50176231
CTCGTAT21100.033.05772843
ATCGGAA21900.032.7967531
TCGTATG21350.032.77548644
TCTCGTA21550.032.36795842
GTCACCG21650.032.3153128
CGTAATC22100.031.66319737
ACACGTC22650.031.48158512
TCGGAAG23100.031.3557032
CGTCTGA22850.031.30447615
CACCGTA22550.031.1263530
TACGTAA23500.029.9680435
ACGTAAT23900.029.74891336
CGGAAGA24750.029.5379813
ATCTCGT23850.029.15297341
ACGTCTG24900.028.81753514
Produced by FastQC (version 0.11.5)