FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4735278
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGC77070.162757075719736TruSeq Adapter, Index 16 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG9750.040.9296434
CTCGTAT10250.039.23629443
CGTATGC10450.038.91495545
TCTCGTA8900.038.62426442
GTCCCGA10200.038.4570435
TCGTATG10550.038.12016744
TCCCGAT9100.036.87654536
CCCGATC9050.036.8297437
ACCCGTC11000.036.7645731
CCGATCT9050.036.57919738
CGTCTGA11200.035.7076115
CACCCGT11400.035.6727930
ACACGTC11400.035.0811612
ATCTCGT9600.034.87138741
CCGTCCC11750.034.227433
ATCGGAA11850.034.138261
TCACCCG11850.033.7503929
CCCGTCC12100.033.42268832
CGGAAGA12200.033.336763
TCGGAAG12150.033.1044242
Produced by FastQC (version 0.11.5)