FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16638809
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG10950.026.70686345
CGATGTA11500.026.01657333
CGTATGC11700.025.37942143
ACCGATG11900.024.76398831
CACCGAT11950.024.66022730
CCGATGT12300.023.95865632
CTCGTAT12500.023.7551441
ATCGGAA12650.023.6660421
TCGGAAG13950.022.1027492
TCTCGTA11400.022.10074240
TATCTCG11550.022.00848438
TCGTATG13950.021.44722642
GTCACCG14300.021.23704128
ACACGTC14650.020.72954812
CGTCTGA15100.020.11177815
ATCTCGT12950.019.97660639
TCACCGA16250.018.68859729
CGGAAGA17450.017.5388183
ACGTCTG18950.016.38187214
CACGTCT20750.015.2860313
Produced by FastQC (version 0.11.5)