FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9663569
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC312440.32331739960670847TruSeq Adapter, Index 11 (100% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCG177460.18363815687558085TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA34300.041.7415121
TATGCCG23650.038.6248145
CTCGTAT55250.025.65638242
ACGGCTA55250.025.53645532
TCTCGTA55400.025.5056941
CGTATGC56150.025.24397344
CGGCTAC55750.025.22670433
ATCTCGT56200.025.10258140
TCGTATG56750.024.97824143
ACACGTC57500.024.92390313
CGTCTGA57200.024.89742316
GTCACGG56950.024.8915529
ATCGGAA58900.024.4120462
AGTCACG57900.024.40566828
TCGGAAG59000.024.3325333
TCACGGC58450.024.21426430
CATCTCG58250.024.14188839
CACGGCT58850.024.0126931
ACGTCTG59900.023.85016415
CGGAAGA61650.023.3961054
Produced by FastQC (version 0.11.5)