FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11141611
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGC201260.18063815008439982TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC27700.038.09609645
ATCGGAA30900.035.1394581
TCGGAAG31350.034.655972
GTCACGA30600.034.4785628
TATCTCG30550.033.9544440
CTCGTAT30650.033.69668243
TCTCGTA31000.033.31638342
TCGTATG31850.033.06159644
ACACGTC32950.032.56323612
CGTCTGA33000.032.51389715
AGTCACG32050.032.49784527
CACGAGT32400.032.4940930
ATCTCGT31950.032.46748441
CGGAAGA33700.032.439553
ACGAGTG33700.031.30820531
ACGTCTG35500.030.22418414
CACGTCT35950.030.03356713
GCACACG37700.028.52010310
CGAGTGG37850.027.99434532
CACACGT40250.027.21620811
Produced by FastQC (version 0.11.5)