FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11805682
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCG680000.5759938307672525TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG77850.042.7168345
ATCGGAA84650.040.17361
CTCGTAT83650.039.7030541
TCTCGTA84050.039.540240
TCGTATG84300.039.3967542
CGTATGC84600.039.31023843
TCGGAAG86750.039.232332
ACACGTC87600.038.77412412
CGTCTGA87050.038.42477415
ATCTCGT86750.038.28296739
CGGAAGA89300.038.1624153
ACGTCTG88200.037.9747814
CATCTCG88050.037.6920338
CACGTCT91050.037.2308113
GCACACG93000.036.6196310
CACACGT94100.036.21530511
GTATGCC92250.036.1220144
GATCATC101850.032.538935
AGTCACC106050.031.67091427
GTCACCA109000.030.89592728
Produced by FastQC (version 0.11.5)