FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5735689
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA3350.031.5359861
TATGCCG2800.028.88715245
CTCGTAT5550.019.83642442
TCTCGTA5650.019.08734341
CGTATGC6050.018.19704844
TATCTCG5750.018.13867839
TCGTATG6250.017.61474243
ATCGGAA6300.017.1215952
ACACGTC6400.016.8506913
ATCTCGT6250.016.6872940
TCGGAAG7100.015.8250043
CGTCTGA6850.015.41571716
ACGTCTG7750.014.49521815
CACGTCT8250.013.88905414
CGGAAGA8350.013.7242754
ACGGGAT9050.011.4198899
CGTTGGA13250.011.3661211
GCACACG9300.011.11300111
GTATGCC10200.011.01361945
CACACGT10400.010.36965712
Produced by FastQC (version 0.11.5)