FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11292697
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG15950.037.944745
CTCGTAT17750.034.2285441
ATCGGAA18450.033.1785241
TCGTATG18450.032.92989342
TCTCGTA17300.032.90739440
CGTATGC18650.032.5767643
TATCTCG17750.031.81958238
CGTCTGA19800.031.58965115
ACACGTC19950.031.12589512
TCGGAAG20050.030.6372682
ATCTCGT18700.030.08274739
ACGTCTG22350.027.88479814
CGGAAGA22350.027.4839553
CACGTCT23600.026.4078513
GCACACG25950.024.27627610
GTATGCC25600.023.72921244
CACTAGC27900.022.50000230
CACACGT28800.021.56145311
GTCACTA30300.020.71791528
ACTAGCT33050.019.12892231
Produced by FastQC (version 0.11.5)