FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6206345
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCG114040.1837474391127145TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG14950.039.88656245
CTCGTAT16900.034.61434641
ATCGGAA17700.034.6072921
ACACGTC17700.034.19223412
CGTATGC17500.033.81408743
TCGTATG17600.033.62141842
TCGGAAG18250.033.4258422
TCTCGTA15450.032.7649540
CGTCTGA18500.032.47068815
CGGAAGA19500.031.7451613
ACGTCTG19750.030.52925114
CACGTCT19950.030.1104213
TCACGAT17100.029.866834
ATCTCGT17850.028.3595839
GCACACG22350.027.48106610
CACACGT22050.027.44682711
CATCACG21900.027.12203632
GTATGCC22000.027.10473444
ACGATCT19150.026.43459736
ATCACGA22700.026.0670833