FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9523919
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA6550.033.559591
CGGCTAC8200.026.61237333
ACGGCTA8550.025.78542332
CTCGTAT9200.024.209342
TCTCGTA8850.023.38738441
CGTATGC9850.022.38403544
ATCGGAA10200.022.0579072
TCGGAAG10450.021.9608123
TCGTATG10200.021.39538443
GTCACGG10700.020.81448629
AGTCACG11150.020.37753728
CGTCTGA11000.019.83643716
ACACGTC11250.019.59558513
ATCTCGT10750.019.25380140
TCACGGC11900.018.71554830
CGGAAGA12850.017.859464
CACGGCT12650.017.7837731
ACGTCTG13500.016.49628315
CATCTCG12500.016.378239
TATGCCG4150.016.26378645
Produced by FastQC (version 0.11.5)