FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11997251
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCG5448814.541715431310056TruSeq Adapter, Index 12 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC1558911.2993893351068506TruSeq Adapter, Index 12 (100% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAAACTCGTATGCCG185890.15494382838201853TruSeq Adapter, Index 12 (98% over 51bp)
GAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTT162660.13558105936101528TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA172250.044.3436051
TATGCCG646650.042.47602545
ACTCGTA23550.036.39489740
ATCGGAA792500.035.3234561
TCGGAAG797550.035.046962
CGGAAGA805250.034.6587523
ACACGTC814950.033.9340112
CGTCTGA815950.033.83452215
CACGTCT819200.033.7497213
ACGTCTG818100.033.74010514
GCACACG823200.033.6753510
CGTATGC817800.033.59214443
CACACGT824650.033.57794611
CTCGTAT814500.033.53793741
TCGTATG818200.033.49323342
TCTCGTA776150.033.32309340
GTATGCC826900.033.2387544
ATCTCGT778150.033.23455439
AGAGCAC842300.033.125397
AATCTCG783700.032.97336238
Produced by FastQC (version 0.11.5)