FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6252546
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCG77610.12412543626228419TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG10500.037.5025745
GATCGGA3650.032.6886371
CTCGTAT12950.030.23083341
CGTATGC13450.029.10724443
TCTCGTA12800.029.00311340
CGATCAG13500.028.99897432
TCGTATG13650.028.68076142
ACACGTC13900.028.64960712
GTCACGA13600.028.12400628
ATCGGAA14050.027.8796121
TCGGAAG14400.027.5100272
ACGATCA13950.027.41838631
ATCTCGT13850.027.12965639
TCACGAT14300.027.0619829
CGTCTGA14800.026.90740215
CGGAAGA14950.026.1951623
AGTCACG15050.025.86287327
ACGTCTG15500.025.83738314
CACGTCT16450.024.7555713
CACGATC16350.023.66907730
Produced by FastQC (version 0.11.5)