FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8976295
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC4600.020.54298845
CTCGTAT4650.019.8412243
ACACGTC5350.017.24200412
ATCGGAA5500.017.1861021
CGTTGGA25600.015.9121691
TCGTATG6000.015.37463444
TCTCGTA5150.015.29319942
GTCACGT6750.015.00022928
AGTCACG6700.014.44051827
ACGGGAT21200.014.1147599
TCGGAAG7250.013.9665852
AAACGGG31400.013.757947
AACGGGA27750.013.7026828
GAAACGG32300.013.5836486
CGTCTGA7050.013.40348815
GGAAACG31750.012.7559585
CGGGATT20600.012.45074410
ATCTCGT6850.012.15468941
ACGTCTG8150.012.14654414
AACGATC6800.011.91287537
Produced by FastQC (version 0.11.5)